Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan
نویسندگان
چکیده
For the first time the presence of dinophysistoxin-1 (DTX-1) in a culture of Prorocentrum foraminosum was revealed in cells and in the culture medium. The clone was isolated from coastal waters of the Sea of Japan and identified by molecular analyses of SSU and D1/D2 regions of LSU rDNA. The concentration of DTX-1 in cells was 8.4 ± 2.5 pg/cell and, in cell-free media, 27.9 ± 14.7 µg/L. The toxin presence was confirmed by HPLC with high-resolution tandem mass-spectrometry.
منابع مشابه
Accumulation and Tissue Distribution of Dinophysitoxin-1 and Dinophysitoxin-3 in the Mussel Crenomytilus grayanus Feeding on the Benthic Dinoflagellate Prorocentrum foraminosum
A DTX-1-producing microalga, Prorocentrum foraminosum, from Peter the Great Bay, Sea of Japan, was fed to Gray's mussels, Crenomytilus grayanus, for 12 days. An increase in DTX-1 and 7-O-acyl-DTX-1 (DTX-3) was observed in the digestive gland, kidneys, and gills. The digestive gland accumulated 91-100% of DTX-1 + DTX-3; and kidneys and gills accumulated, up to 8.5% and 4.3%, respectively. The ki...
متن کاملChecklist of Phytoplankton Taxa in the Iranian Waters of the Caspian Sea
Phytoplankton taxa composition of the Caspian Sea were evaluated by using samples collected at 12 stations along the Iranian coast of the Caspian Sea between 1996 and 2010. This study identified 158 phytoplankton species consisted of diatoms (70 species), dinoflagellates (23 species), chlorophytes (29 species), cyanophytes (31 species), and euglenoids (5 species). The dominant species were the...
متن کاملProfiling of Extracellular Toxins Associated with Diarrhetic Shellfish Poison in Prorocentrum lima Culture Medium by High-Performance Liquid Chromatography Coupled with Mass Spectrometry
Extracellular toxins released by marine toxigenic algae into the marine environment have attracted increasing attention in recent years. In this study, profiling, characterization and quantification of extracellular toxin compounds associated with diarrhetic shellfish poison (DSP) in the culture medium of toxin-producing dinoflagellates were performed using high-performance liquid chromatograph...
متن کاملNovel Approaches to Molecular Diagnostics of Prorocentrum Minimum in the Golden Horn Estuary, the Sea of Marmara
The identification and quantification of individual algal species remains difficult, time consuming, requiring a great deal of expertise and experience. Alternative to traditional, morphology-based methods, molecular techniques need less expertise for the detection of cryptic species within the bioindicator community. Although these methods require detailed knowledge of the molecular diversity ...
متن کاملPalynomorphs’ response to sea-level fluctuations: a case study from Late Cretaceous – Paleocene, Gurpi Formation, SW Iran
Statistical studies on Palynology contents of Late Cretaceous to Paleocene age Gurpi Formation in a surface section in Zagros Basin,SW Iran indicate changes in abundance, species diversity, ratio of Spiniferites to Cyclonephelium (S/C), palynological marine index(PMI) values and organic facies. These palynological variations clearly reflect fluctuations in relative sea-level and depositionalenv...
متن کامل